June 25, 2024 @ 9:30 A.m. (JST)

June 25 @ 2:30 AM (CEST) / June 24 @ 5:30 PM (PDT) / June 24 @ 7:30 PM (CST) 


Dr. Philipp T. Kaulich

(Christian-Albrechts-Universität Kiel, Germany)


Dr. Luca Fornelli

(Oklahoma University, U.S.A.) 


Dr. Takashi Baba 

(SCIEX, Canada)


Dr. Nobuaki Takemori

(Ehime University, Japan) 

About the webinar


Welcome to the Third Annual JPrOS Webinar Series “Top-Down Proteomics”! In our 2024 webinar, leading researchers in the cutting-edge field of top-down proteomics, will discuss the some of the latest exciting developments around the world. The webinar series has been designed to appeal not only to researchers but also to participants who want to learn about top-down proteomics, which is still not widely used in Japan. All lectures are easy to understand and require no prior knowledge. We hope that attending this webinar will be a wonderful opportunity to learn the fun of intact proteoform research and to start top-down proteomics in your own laboratories!

Sample Preparation for Top-Down Proteomics

Dr. Philipp T. Kaulich
Dr. Philipp T. Kaulich

In addition to mass spectrometry techniques, the choice of an appropriate sample preparation method is critical for top-down proteomics. But there are a variety of sample preparation methods for intact proteoforms. Which one should you use? Dr. Kaulich gave a very clear answer to this difficult question at this year’s ASMS conference in Anaheim just a few weeks ago. Join us for this webinar to learn about the best sample preparation methods available today!





References for this lecture

Influence of Different Sample Preparation Approaches on Proteoform Identification by Top-Down Proteomics.

Kaulich PT, Jeong K, Kohlbacher O, Tholey A.

PREPRINT (Version 1) available at Research Square. 2024 Mar 20. https://doi.org/10.21203/rs.3.rs-3990966/v1.


Improved Identification of Proteoforms in Top-Down Proteomics Using FAIMS with Internal CV Stepping.

Kaulich PT, Cassidy L, Winkels K, Tholey A.

Anal Chem. 2022 Mar 1;94(8):3600-3607. doi: 10.1021/acs.analchem.1c05123.


Intact Proteoform Analysis by Top-Down Mass Spectrometry

Dr. Luca Fornelli
Dr. Luca Fornelli

"I'm interested in top-down proteomics, but I’ll need special mass spectrometry equipment, right?" This is a question we often get from JPrOS members. Dr. Fornelli is a true expert who has successfully analyzed many intact proteoforms using bench-top Orbitrap mass spectrometers which many of you already have access to. In his talk, you will get insights into new proteoform analysis techniques using state-of-the-art mass spectrometry, as well as practical advice on how to start top-down proteomics studies in your own laboratory.





References for this lecture

Targeted Quantification of Proteoforms in Complex Samples by Proteoform Reaction Monitoring.

Huang CF, Kline JT, Negrão F, Robey MT, Toby TK, Durbin KR, Fellers RT, Friedewald JJ, Levitsky J, Abecassis MMI, Melani RD, Kelleher NL, Fornelli L.

Anal Chem. 2024 Feb 27;96(8):3578-3586. doi: 10.1021/acs.analchem.3c05578.


Orbitrap Mass Spectrometry and High-Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS) Enable the in-Depth Analysis of Human Serum Proteoforms.

Kline JT, Belford MW, Boeser CL, Huguet R, Fellers RT, Greer JB, Greer SM, Horn DM, Durbin KR, Dunyach JJ, Ahsan N, Fornelli L.

J Proteome Res. 2023 Nov 3;22(11):3418-3426. doi: 10.1021/acs.jproteome.3c00488.

Mass Spectrometry-Based Proteoform Characterization

Dr. Takashi Baba
Dr. Takashi Baba

To accurately identify intact proteoforms by mass spectrometry, it is important not only to obtain high-resolution MS information from large protein molecules, but also to dissociate their ions into fragments for determining their post-translational modifications and sequences. Dr. Baba will give a highly informative lecture covering everything from the principles of proteoform identification by mass spectrometry to the latest dissociation methods for top-down proteomics.





References for this lecture

Analysis of Sialic Acid Linkage in N-Linked Glycopeptides Using Liquid Chromatography-Electron-Activated Dissociation Time-of-Flight Mass Spectrometry.

Liu S, Ryumin P, Albanese J, Zhang Z, Baba T.

Anal Chem. 2023 May 16;95(19):7458-7467. doi: 10.1021/acs.analchem.2c04581.


Localization of Multiple O-Linked Glycans Exhibited in Isomeric Glycopeptides by Hot Electron Capture Dissociation.

Baba T, Zhang Z, Liu S, Burton L, Ryumin P, Le Blanc JCY.

J Proteome Res. 2022 Oct 7;21(10):2462-2471. doi: 10.1021/acs.jproteome.2c00378.

time table

Time (JST)  
9:30 – 9:40 Opening Remarks (Nobuaki Takemori)
9:40 – 10:20 Influence of Different Sample Preparation Approaches on Proteoform Identification by Top-Down Proteomics (Philipp T. Kaulich)
10:20 – 11:00 Enhanced Sequencing of Intact Proteins and Their Subunits using Ion-Ion and Ion-Photon Reactions in the Gas-Phase (Luca Fornelli)
11:00 – 11:40 Electron Activated Dissociation for Protein Characterization (Takashi Baba)

register now!!

This zoom webinar is free of charge and open to all, including non-members!


A link to participate in the JPrOS webinar will be sent to your registered e-mail address. If you do not receive the link by June 23, please contact us.


For inquiries about the webinar: takemori(at)m.ehime-u.ac.jp  


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